Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: CD11b/c Biotin (OX-42) , CD11b/c, CR3 , Cedarlane , CL042B , 2 μg/ml.

Techniques: Concentration Assay, Flow Cytometry, Control, Staining, FACS

Journal: PLoS ONE

Article Title: Syntaxin Binding Protein 1 Is Not Required for Allergic Inflammation via IgE-Mediated Mast Cell Activation

doi: 10.1371/journal.pone.0058560

Figure Lengend Snippet: A, Wild type, STXBP1 mutant, or heterozygous mice were identified by PCR using DNA from liver cells. B , After 4–5 weeks of culture, liver-derived mast cells (LMC) were examined by toluidine blue staining to determine mast cell purity. A representative photomicrograph is shown (original magnification = ×100). C , LMCs were sensitized with anti-TNP IgE, stained with FITC-conjugated anti-IgE or anti-c-kit antibodies, and examined by flow cytometry. D, STXBP1 +/+ and STXBP1 −/− LMCs were sensitized with anti-TNP IgE and stimulated with TNP-BSA (10 ng/ml) for various times. Whole cell lysates were analyzed by Western blotting for STXBP1 and Actin. E, Sec1/Munc18 (SM) family gene expression profiles were identified by RT-PCR using mRNA from wild type mouse bone marrow-derived mast cells (BMMC), and wild type (STXBP1 +/+ ) or STXBP1 −/− LMCs, with or without TNP-BSA stimulation. All members of SM family are expressed in wild type BMMCs and LMCs. Similar results were observed in 2 independent experiments.

Article Snippet: FITC-conjugated rat anti-mouse IgE (IgG1), FITC-rat IgG1 and FITC-conjugated rat anti-mouse CD117 (c-kit) were purchased from Cedarlane Laboratories (Burlington, ON, Canada).

Techniques: Mutagenesis, Derivative Assay, Staining, Flow Cytometry, Western Blot, Gene Expression, Reverse Transcription Polymerase Chain Reaction

Journal:

Article Title: A cholera toxoid-insulin conjugate as an oral vaccine against spontaneous autoimmune diabetes

doi:

Figure Lengend Snippet: T cells from CTB-insulin-fed NOD mice suppress beta cell infiltration by diabetogenic T cells. Immunofluorescence staining of pancreatic islets from irradiated NOD males 30 days after co-injection of syngeneic Thy-1,2+ T cells from 20-week-old diabetic female mice with Thy-1,1+ T cells from congenic NOD mice that were fed CTB-insulin (A, C) or CTB-ovalbumin (B, D). Immunostainings were performed with monoclonal antibodies to Thy-1,2+ (A, B) and Thy-1,1+ (C, D). Original magnifications ×250.

Article Snippet: The percentage of donor Thy-1,1+ T cells in the spleen, mesenteric, and pancreatic lymph nodes of recipient NOD males was determined 7, 15, and 30 days after cell transfer by cytofluorometric analysis using FITC-labeled rat monoclonal antibodies to Thy-1,1 (clone CL005F; Cedarlane Laboratories) or Thy-1,2 (clone 30H12; Biosys, Compiègne, France) ( 32 ).

Techniques: Immunofluorescence, Staining, Irradiation, Injection

Journal: Scientific Reports

Article Title: Graphene Oxide-Gold Nanosheets Containing Chitosan Scaffold Improves Ventricular Contractility and Function After Implantation into Infarcted Heart

doi: 10.1038/s41598-018-33144-0

Figure Lengend Snippet: Implantation of conductive polymer patch did not induce host immune response in vivo : ( A , B ) Host immune response against implanted scaffold was assessed by evaluating CD4 + and CD8 + T cell infiltration in the myocardium by immunohistochemistry. The scaffold implantation did not cause any significant change in the number of infiltrating CD4 + and CD8 + T cells in the myocardium compared to MI group. *P < 0.05 compared to sham group. (n = 5). Data is expressed as mean ± SD.

Article Snippet: After removing the wax, sections were rehydrated using different concentrations of ethanol (100%, 95%, 70%, 50%) and washed with 1X PBS for 10 min. After blocking with 1% horse serum for 30 min, sections were incubated with anti-CD4 or anti-CD8 antibody (Cedarlane, CL003AP, CL004AP) at 1:100 dilution overnight at 4 °C.

Techniques: Polymer, In Vivo, Immunohistochemistry